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- Title
A comparison between constitutive and inducible transgenic expression of the PhRIP I gene for broad-spectrum resistance against phytopathogens in potato.
- Authors
Gonzales-Salazar, Romel; Cecere, Bianca; Ruocco, Michelina; Rao, Rosa; Corrado, Giandomenico
- Abstract
Objectives: To engineer broad spectrum resistance in potato using different expression strategies. Results: The previously identified Ribosome-Inactivating Protein from Phytolacca heterotepala was expressed in potato under a constitutive or a wound-inducible promoter. Leaves and tubers of the plants constitutively expressing the transgene were resistant to Botrytis cinerea and Rhizoctonia solani, respectively. The wound-inducible promoter was useful in driving the expression upon wounding and fungal damage, and conferred resistance to B. cinerea. The observed differences between the expression strategies are discussed considering the benefits and features offered by the two systems. Conclusions: Evidence is provided of the possible impact of promoter sequences to engineer BSR in plants, highlighting that the selection of a suitable expression strategy has to balance specific needs and target species.
- Subjects
RIBOSOME-inactivating proteins; PHYTOLACCA; TRANSGENES; BOTRYTIS cinerea; RHIZOCTONIA solani
- Publication
Biotechnology Letters, 2017, Vol 39, Issue 7, p1049
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-017-2335-0