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- Title
High pressure treatment under subfreezing temperature results in drastic inactivation of enveloped and non-enveloped viruses.
- Authors
Kishida, T.; Cui, F.-D.; Ohgitani, E.; Gao, F.; Hayakawa, K.; Mazda, O.
- Abstract
Some viruses are sensitive to high pressure. The freeze-pressure generation method (FPGM) applies pressure as high as 250 MPa on a substance, simply by freezing a pressure-resistant reservoir in which the substance is immersed in water. Here we examined whether the FPGM successfully inactivates herpes simplex virus type 1 (HSV-1), an enveloped DNA virus belonging to the human Herpesviridae, and encephalomyocarditis virus (EMCV), an envelope-free RNA virus belonging to the Picornaviridae. After the treatment, HSV-1 drastically reduced the ability to form plaque in Vero cells in vitro as well as to kill mice in vivo. EMCV that had been pressurized failed to proliferate in HeLa cells and induce interferon response. The results suggest that the FPGM provides a feasible procedure to inactivate a broad spectrum of viruses.
- Subjects
HIGH pressure biotechnology; VIRUS inactivation; DNA viruses; ENCEPHALOMYOCARDITIS virus; PICORNAVIRUSES; CELL proliferation
- Publication
Biotechnology Letters, 2013, Vol 35, Issue 8, p1297
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1204-8