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- Title
Secretory production of cell wall components by Saccharomyces cerevisiae protoplasts in static liquid culture.
- Authors
Aoyagi, Hideki; Ishizaka, Mikiko; Tanaka, Hideo
- Abstract
When protoplasts of Saccharomyces cerevisiae T7 and IFO 0309 are cultured in a static liquid culture at 2.5 × 10 protoplasts/ml, cell wall regeneration does not occur and cell wall components (CWC) are released into the culture broth. By using a specialized fluorometer, the concentrations of CWC could be measured on the basis of the fluorescence intensity of the CWC after staining with Fluostain I. The inoculum concentration, pH, and osmotic pressure of the medium were important factors for the production of CWC in culture. Under optimal culture conditions, S. cerevisiae T7 protoplasts produced 0.91 mg/ml CWC after 24 h. The CWC induced the tumor necrosis factor-α production about 1.3 times higher than that of the commercially available β-1,3/1,6-glucan from baker's yeast cells.
- Subjects
BACTERIAL cell walls; MICROBIAL protoplasts; SACCHAROMYCES cerevisiae; REGENERATION (Biology); FLUORIMETER; MICROBIAL cultures; YEAST fungi biotechnology
- Publication
Biotechnology Letters, 2012, Vol 34, Issue 4, p695
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-011-0817-z