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- Title
Doku tiplendirme laboratuvarına gönderilmiş olan örneklerde HLA antijenlerinin dağılımının araştırılması.
- Authors
Mete, Ergun
- Abstract
Purpose: In this study, it was aimed to determine the frequency of HLA class I and class II alleles and to evaluate the results of HLA genotyping in people who applied to the Health Research and Application Center Tissue Typing Laboratory. Material and method: In our study, the data of 1903 patients were examined and the HLA class I and II allele frequencies were calculated. HLA A, B and DRB1 alleles were studied in 1903 individuals, HLA C and DQB1 alleles were studied in 493 individuals. 57.4% of the subjects were male and 42.3% were female. Genomic DNA isolation from whole blood samples was performed using an automated system (EZ1 Advanced, QIAGEN, Hilden, Germany). For the determination of HLA alleles, PCR-SSO (squence specific oligonucleotide) method and Luminex 200 system (Luminex, Austin, USA) were used. PCR-SSO (squence specific oligonucleotide) method was used to identify HLA alleles. Results: The most common HLA-A alleles were HLA-A*02 (22.3%), HLA-A*24 (17.1%), and HLA-A*03 (10.5%). The most common HLA-B alleles were HLA-B*35 (16.3%), B*51 (15.2%), and B*44 (8.3%). HLA-C alleles; HLA-C*04 (19.1%), HLA-C*07 (16.0%), and HLA-C*12 (15.0%). HLA-DRB1 alleles; HLA-DRB1*11 (19.9%), HLA-DRB1*04 (17.7%), and HLA-DRB1*15 (10.5%). HLA-DQB1 alleles; HLA-DQB1*03 (40.9%), HLA-DQB1*05 (25.7%), and HLA-DQB1*06 (18.3%). Conclusion: Our findings are mostly consistent with some differences when compared with HLA antigens identified in other studies conducted in our country. This study will contribute to the HLA allele frequency data and the determination of HLA diversity in our region.
- Publication
Pamukkale Medical Journal, 2021, Vol 14, Issue 3, p742
- ISSN
1309-9833
- Publication type
Article
- DOI
10.31362/patd.943791