We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Resistance in the mosquito, Culex quinque-fasciatus, and possible mechanisms for resistance.
- Authors
Qiang Xu; Huqi Liu; Lee Zhang; Nannan Liu
- Abstract
Two mosquito strains of Culex quinquefasciatus (Say), MAmCqG0 and HAmCqG0, were collected from Mobile and Huntsville, Alabama, respectively. MAmCqG0 and HAmCqG0 were further selected in the laboratory with permethrin for one and three generations, respectively. The levels of resistance to pennethrin in MAmCqG1 (after one-generation selection) and HAmCqG3 (after three-generation selection) increased rapidly. Resistance to permethrin in MAmCqG1 and HAmCqG3 was partially suppressed by piperonyl butoxide (PBO), S,S,S-tributylphosphorotrithioate (DEF) and diethyl maleate (DEM), inhibitors ofcytochrome P450 monooxygenases, hydrolases and glutathione S-transferases (GST), respectively, suggesting these three enzyme families are important in conferring permethrin resistance in both strains. A substitution of leucine to phenylalanine (L to F) resulting from a single nucleotide polymorphism (SNP), termed the kdr mutation, in the parashomologous sodium channel gene has been reported as a very common mutation associated with pyrethroid resistance of insects. A 341-bp sodium channel gene fragment, where the kdr mutation resides, was generated by PCR from genomic DNAs of Cx. quinquefasciatus strains. We found that the kdr mutation was present in both permethrin-selected and unselected HAmCq and MAmCq mosquito populations, suggesting that the hdr mutation plays the role in pennethrin resistance. There was no significant change in the frequency and heterozygosity of the A to T SNP for the kdr allele between permethrin-selected and unselected MAmCq and HAmCq mosquitoes, indicating that other mechanisms are involved in the evolution of resistance in mosquitoes selected by permethrin in the laboratory.
- Subjects
INSECTICIDE resistance; MOSQUITOES; INSECTICIDES; ENZYMES; ION channels; POLYMORPHISM (Zoology)
- Publication
Pest Management Science, 2005, Vol 61, Issue 11, p1096
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.1090