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- Title
Placental growth factor blunts uterine artery responses to angiotensin II.
- Authors
Espinoza, J; Betancourt, A; Belfort, MA; Shamshirsaz, AA; Fox, KA; Yallampalli, C; Belfort, M A; Shamshirsaz, A A; Fox, K A
- Abstract
<bold>Objective: </bold>Changes in maternal serum concentration of placental growth factor (PlGF) and vascular response to intravascular infusion of Angiotensin II (Ang II) follow a bell-shaped curve pattern during gestation. This study evaluates the effects of PlGF and soluble vascular endothelial growth factor receptor-1 (sFlt-1) on responses of human uterine arteries (UA) to Ang II.<bold>Design: </bold>Experimental.<bold>Setting: </bold>Baylor College of Medicine and Texas Children's Hospital-Pavilion for Women.<bold>Sample: </bold>Uterine arteries samples (n = 14) were obtained from normotensive women undergoing caesarean hysterectomy at ≥32 weeks.<bold>Methods: </bold>Uterine arteries rings were incubated with (1) Krebs solution; (2) PlGF at 1.45, 14.5, and 500 pg/ml; (3) sFlt-1 at 2 ng/ml; and (4) a combination of sFlt-1, and PlGF. Dose-contraction responses to Ang II were determined in UA rings incubated in the above-mentioned conditions. Responses were also measured in presence of L-NAME or inhibitors of endothelium-derived hyperpolarising factor: apamine and charybdotoxin. The t-test was used for comparisons.<bold>Main Outcome Measure: </bold>Changes in vascular reactivity of UA rings.<bold>Results: </bold>PlGF blunted (P = 0.03) and sFlt-1 increased (P <0.01) the UA maximum responses to Ang II. A combination of sFlt-1 and PlGF blunted UA responses to Ang II (P < 0.05). l-NAME, apamine, and charybdotoxin reversed the relaxation effects of PlGF on UA responses to Ang II (P < 0.05).<bold>Conclusions: </bold>PlGF contributes to the blunted vascular response to Angiotensin II during normotensive pregnancies and sFlt-1 appears to attenuate this effect. PlGF and sFlt-1 may contribute to the regulation of vascular tone during pregnancy by altering the vascular response to Angiotensin II.<bold>Funding: </bold>Baylor College of Medicine.<bold>Tweetable Abstract: </bold>Placental growth factor and soluble vascular endothelial growth factor receptor-1 modulate the uterine artery response to Angiotensin II in normotensive pregnant women.
- Subjects
ARTERIES; BLOOD pressure; CELL receptors; RESEARCH funding; VASOCONSTRICTORS; ANGIOTENSIN II; PHARMACODYNAMICS
- Publication
BJOG: An International Journal of Obstetrics & Gynaecology, 2019, Vol 126, Issue 8, p1058
- ISSN
1470-0328
- Publication type
journal article
- DOI
10.1111/1471-0528.15656