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- Title
Genetic analysis of shiga-toxigenic Escherichia coli isolates from cattle in a limited region.
- Authors
Otawa, Kenichi; Sato, Masaaki; Sasaki, Takako; Sasaki, Hiraku; Nonaka, Jun; Ito, Kikuji; Kuroki, Toshio; Nakai, Yutaka
- Abstract
The ecology of shiga-toxigenic Escherichia coli (STEC) is important in the animal production environment. We investigated fecal shedding of STEC in one town in Miyagi, Japan by multiplex polymerase chain reaction (PCR) targeting shiga toxin gene 1 ( stx 1), gene 2 ( stx 2) and malB promoter gene, and analyzed the PCR products of stx 1 or stx 2 (54 samples) by direct sequencing. Three of 46 (6.5%) beef cattle in the University Farm of Tohoku University (Kawatabi Farm) and 11 of 70 (15.7%) calves in neighboring dairy farms carried STEC. Rate of detecting genes of stx 1, stx 2 and stx 1+2 was 3.4% (4/116), 8.6% (10/116) and 0.9% (1/116), respectively. Serotyping indicated that STEC contaminated farms at different times or through different routes. Isolates harbored no mutation among stx 1, but six (Kawatabi Farm) and 38 (neighboring farms) base substitutions among stx 2, respectively. The diversity of substitutions of stx 2 was observed among farms or even in a farm. Phylogenic analysis revealed that STEC detected in the area were classified into three clusters by the variety of stx 2. Sequence analysis of stx 2 will be one of the tools for clarifying the source of outbreaks and the route of contamination of STEC.
- Subjects
CATTLE; EPIDEMIOLOGY; GENES; ESCHERICHIA coli; GENETICS
- Publication
Animal Science Journal, 2004, Vol 75, Issue 3, p261
- ISSN
1344-3941
- Publication type
Article
- DOI
10.1111/j.1740-0929.2004.00185.x