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- Title
The Pentratricopeptide Repeat Protein Pigment-Defective Mutant2 is Involved in the Regulation of Chloroplast Development and Chloroplast Gene Expression in Arabidopsis.
- Authors
Liang Du; Jian Zhang; Shaofeng Qu; Yuanyuan Zhao; Bodan Su; Xueqin Lv; Ruili Li; Yinglang Wan; Jianwei Xiao
- Abstract
The development of functional chloroplasts, which is assisted by a series of nuclear-encoded auxiliary protein factors, is essential for plant autotrophic growth and development. To understand the molecular mechanisms underlying chloroplast development, we isolated and characterized a pigment-defective mutant, pdm2, and its corresponding variegated RNA interference (RNAi) lines in Arabidopsis. Sequence analysis revealed that PDM2 encodes a pentatricopeptide repeat protein that belongs to the P subgroup. Confocal microscopic analysis and immunoblotting of the chloroplast protein fraction showed that PDM2 was located in the stroma. In RNAi plants, protein-related photosynthesis was severely compromised. Furthermore, analysis of the transcript profile of chloroplast genes revealed that plastid-encoded polymerase-dependent transcript levels were markedly reduced, while nuclearencoded polymerase-dependent transcript levels were increased, in RNAi plants. In addition, PDM2 affects plastid RNA editing efficiency in most editing sites, apparently by directly interacting with multiple organellar RNA editing factor 2 (MORF2) and MORF9. Thus, our results demonstrate that PDM2 is probably involved in the regulation of plastid gene expression required for normal chloroplast development.
- Subjects
CHLOROPLASTS; PLANT development; PLANT molecular systematics; PLANT molecular phylogenetics; RNA interference; GENE expression in plants; PLANT molecular genetics
- Publication
Plant & Cell Physiology, 2017, Vol 58, Issue 4, p747
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pcx004