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- Title
Ultrasonic-assisted aqueous two-phase extraction of polysaccharide from loquat leaves by Box-Behnken response surface optimization and its antioxidant activity.
- Authors
YU Lan; XIAO Yun; CHEN Yan-meng; GUO Song
- Abstract
Box-Behnken design response surface methodology was used to optimize the ultrasonic-assisted aqueous twophase extraction of loquat leaves polysaccharide. The effects of ultrasonic time, ultrasonic power, ultrasonic temperature, and liquid-material ratio on the extraction rate of loquat leaves polysaccharide and the antioxidant activity of loquat leaves polysaccharide were studied. The results showed that the optimum conditions for extracting polysaccharide from loquat leaves by ultrasonic-assisted aqueous two-phase method were ultrasonic time 60 min, ultrasonic power 180 W, ultrasonic temperature 60 °C, liquid-material ratio 70 mL/g, and the yield of polysaccharide from loquat leaves reached 4.222%. The antioxidant activity test showed that the DPPH radical scavenging rate was 73.90% and the hydroxyl radical scavenging rate was 58.45% when the concentration of loquat leaves polysaccharide was 1.2 g/L. The study indicates that the extraction technology of loquat leaves polysaccharide is environmentally friendly and feasible, and loquat leaves polysaccharide have good antioxidant activity.
- Subjects
LOQUAT; POLYSACCHARIDES; RESPONSE surfaces (Statistics); ULTRASONIC effects; HYDROXYL group; ANTIOXIDANT testing
- Publication
Feed Research, 2024, Vol 47, Issue 7, p94
- ISSN
1002-2813
- Publication type
Article
- DOI
10.13557/j.cnki.issn1002-2813.2024.07.017