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- Title
Chymotrypsin im Stuhl: Vergleich der titrimetrischen und photometrischen Methode bei Kindern und Erwachsenen.
- Authors
Appel, W.; Jesper, M.; Appel, S.; Kirstein, l.
- Abstract
The activity of fecal Chymotrypsin was determined by kinetic titration (pH-stat) with Nα-benzoyl-L-argininethylester (reference method) and photometrically with Na-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanin- p-nitranilid (routine method). The photometric fest was carried out with selfmade reagent Solutions and with Monotest®-Chymotrypsin. Solubilisation was achieved in most cases by lauryltrimethylammoniumchloride. 453 specimen ofstool of334 adults, children and newborns have been analyzed. Both kinds of-photometric methods behave equally. Homogenization, extraction, Separation and stability of the enzyme in the extract are satisfying. This also is true when using sample-processing unit developed by the manufacturer. According to the ränge of concentration the imprecision in serie shows CV's of 3-12%, day-to-day (with control material) 1.5-6%. Linearity of Signals up to 30min and 30 U/g stool are given. Comparison of titrimetric (x) and photometric (y) determination by regression analysis (2 collectives in 2 years) result in y = - 0.39 (2.30) + 0.36 (0.40)x, r = 0.793 (0.839), n = 107 (adults); y = - 0.47 + 0.41 xf r = 0.872, n = 92 (children); and y = 0.11 + 7.03x, r = 0.441, n = 43 (newborn). The technical sensitivity and specifity are satisfying. Control material may be used. Newborns with cystic fibrosis showed values of 1-7 U/g (photometry). The preliminary normal borderline for the photometric method for adults and children should be elevated up to 4 U/g. The values obtained with the kit Monotest®-Chymotrypsin with sample processing unit prove the values found by the titrimetric method.
- Publication
Journal of Laboratory Medicine / Laboratoriums Medizin, 1985, Vol 9, Issue 4, p133
- ISSN
0342-3026
- Publication type
Article