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- Title
Measurement results of Mal d 1 protein in apples cultivated in Nagano prefecture, Japan.
- Authors
Naoko Okishima; Kyoka Kawai; Kurumi Kobayashi; Kazumi Tsuruta
- Abstract
Pollen-food allergy syndrome (PFAS) associated with birch and alder pollinosis is becoming a problem in Japan, therefore, it is necessary to find apple cultivars in which Mal d 1, a major PFAS allergen component, is expressed at low levels. Relative quantification of Mal d 1 mRNA in 28 Japanese apple cultivars was performed, and several cultivars with low Mal d 1 expression were identified. As it is the protein and not mRNA that is the allergen, it was necessary to ascertain protein levels. To do this, an ELISA test was constructed for the quantification of Mal d 1 protein levels in apples cultivated in the Nagano prefecture. Mal d 1 extraction solutions were compared, and extraction efficiency was shown to be optimal with minimal sample variation when a composition of 1 mol/L sucrose, 2% polyvinylpolypyrrolidone (PVPP), 2 mmol/L EDTA, and 1 mmol/L diethyldithiocarbamic acid was used. Using this approach, Mal d 1 extraction and quantification were performed on 26 apple cultivars from Nagano prefecture. Of the cultivars tested, 24 were significantly lower in Mal d 1 protein than Sun fuji apples. These low Mal d 1 protein expression cultivars are proposed candidates for producing low-allergenic Japanese apples.
- Subjects
JAPAN; GENE expression; PROTEINS; PROTEIN expression; FLUOROALKYL compounds; ALLERGIC rhinitis; ALNUS glutinosa
- Publication
Japanese Journal of Food Chemistry & Safety, 2023, Vol 30, Issue 1, p1
- ISSN
1341-2094
- Publication type
Article