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- Title
钙敏感受体在大鼠脑缺血再灌注损伤时细胞凋亡中的作用.
- Authors
李凤晓; 史雪莹; 初向华; 卢晓东; 董加花; 于良健; 张宁
- Abstract
Objective: To investigate the effect of calcium sensing receptor on cerebral ischemia-reperfusion (I/R)in rats. Methods: Sixty male Wistar rats, weighing 250-300 g, were randomly divided into three groups(n=20 each)using a random number table: sham operation group(group S), group I/R and calcium-sensing receptor antagonists group (group N). Animal models of middle cerebral artery occlusion/reperfusion in the later 2 groups were established by a filament method in the left external-internal carotid artery. Rats were injected with 1 mg/kg NPS-89636 in the calcium-sensing receptor antagonists group and the equal volume of dimethyl sulfoxide 10 minutes before the cerebral ischemia. Neurological function was assessed and scored at 24 h of reperfusion. The rats were sacrificed and then the brains were removed for determination of MDA content, SOD activity, apoptotic cell death was assessed by TUNEL staining, and the expression of Caspase-3 by immunohistochemical and Western blot. Results: The content of MDA, neuronal apoptosis index, expression levels of Caspase-3 positive cells and Caspase-3 protein in group I/R and N were higher than those in group S, and neurological function score and SOD activity were lower than those in group S(P<0.05); The content of MDA, neuronal apoptosis index, expression levels of Caspase-3 positive cells and Caspase-3 protein in group N were lower than those in group I/R, and neurological function score and SOD activity were higher than those in group I/R (P<0.05). Conclusion: Calcium-sensing receptor is involved in cerebral ischemia-reperfusion injury and cell apoptosis.
- Subjects
CALCIUM-sensing receptors; APOPTOSIS; CELL death; CAROTID artery; DIMETHYL sulfoxide; MYOCARDIAL reperfusion
- Publication
Progress in Modern Biomedicine, 2020, Vol 20, Issue 6, p1043
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2020.06.009