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- Title
Improved Method for n-Alkanes and n-Methyl-Ketones Determination in Litters and Soils.
- Authors
Zavgorodnyaya, Yu. A.; Anokhina, N. A.; Bogatyrev, L. G.; Demin, V. V.
- Abstract
A new technique to extract lipid biomarkers (n-alkanes and n-methyl-ketones) from forest litters and soils using the accelerated solvent extraction (ASE) method was developed. The maximum output of n-alkanes and n-methyl-ketones was obtained by sequential extraction using the chloroform : methanol mixture [3 : 1 (vol)] in the first two extraction cycles and chloroform in the subsequent two cycles. The extraction has been carried out at the temperature of 100°С, pressure of 10.3 MPa, and static extraction time of 5 min. The repeated chloroform treatment by ASE was shown to increase the output of long-chain n-alkanes and n-methyl-ketones.
- Subjects
ALKANES; FOREST litter; SOLVENT extraction; SOILS; PLANT litter; CHLOROFORM
- Publication
Eurasian Soil Science, 2020, Vol 53, Issue 3, p310
- ISSN
1064-2293
- Publication type
Article
- DOI
10.1134/S1064229320030114