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- Title
FcεRII on T cells and IgE-binding factors in children with atopic asthma.
- Authors
Matsumoto, T.; Miike, T.; Takahashi, H.; Hosoda, M.; Kawabe, T.; Yodoi, J.; Hirashima, M.
- Abstract
Expression of low-affinity receptors for IgE (FcεRII) on T and B cells was examined with monoclonal antibodies to cell surface antigens of FcεRII (CD23), T cells (CD3) or B cells (CD19) by two-dimensional analysis under laser flow cytometry system. The majority of cells bearing FcεRII were B cells; however, a small proportion of T cells (1.6%) bore FcεRII in children with atopic asthma and elevated serum level of IgE. The occurrence of FcεRII positive B cells was also increased in children with atopic asthma compared with non-atopic controls (p < 0.01). The numbers of T and B cells bearing Fc receptors for IgG (FcϒR) did not differ between atopic and non-atopic children. T cells were isolated from venous blood by rosetting with sheep erythrocytes, and then cultured in vitro with phytohemagglutinin plus phorbol-myristate-acetate. The amount of IgE-binding factors (IgE-BF), a soluble form of FcεRII, was determined by means of enzyme-linked immunosorbent assay. The level of IgE-BF was increased in cultures from children with atopic asthma compared with non-atopic controls (p < 0.01). The possible involvements of FcεRII on T cells and their soluble products, IgE-BF, in the pathogenesis of atopic asthma is discussed.
- Subjects
IMMUNOGLOBULIN E; B cells; T cells; MONOCLONAL antibodies; ANTIGENS
- Publication
Pediatric Allergy & Immunology, 1990, Vol 1, Issue 1, p21
- ISSN
0905-6157
- Publication type
Article
- DOI
10.1111/j.1399-3038.1990.tb00004.x