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- Title
Preclinical Study of Locoregional Therapy of Hepatocellular Carcinoma by Bioelectric Ablation with Microsecond Pulsed Electric Fields (μsPEFs).
- Authors
Chen, Xinhua; Ren, Zhigang; Jiang, Jianwen; Zheng, Shusen; Li, Chengxiang; Guo, Fei; Zhou, Dianbo; Yao, Chenguo; Chen, Xinmei; Sun, Jihong
- Abstract
Unresectable hepatocellular carcinoma (HCC) needs locoregional ablation as a curative or downstage therapy. Microsecond Pulsed Electric Fields (μsPEFs) is an option. A xenograft tumor model was set up on 48 nude mice by injecting human hepatocellular carcinoma Hep3B cells subcutaneously. The tumor-bearing mice were randomly divided into 3 groups: μsPEFs treated, sham and control group. μsPEFs group was treated by μsPEFs twice in 5 days. Tumor volume, survival, pathology, mitochondria function and cytokines were followed up. μsPEFs was also conducted on 3 swine to determine impact on organ functions. The tumors treated by μsPEFs were completely eradicated while tumors in control and sham groups grew up to 2 cm3 in 3 weeks. The μsPEFs-treated group indicated mitochondrial damage and tumor necrosis as shown in JC-1 test, flow cytometry, H&E staining and TEM. μsPEFs activates CD56+ and CD68+ cells and inhibits tumor proliferating cell nuclear antigen. μsPEFs inhibits HCC growth in the nude mice by causing mitochondria damage, tumor necrosis and non-specific inflammation. μsPEFs treats porcine livers without damaging vital organs. μsPEFs is a feasible minimally invasive locoregional ablation option.
- Subjects
LIVER cancer; ELECTRIC field therapy; ABLATION techniques; XENOGRAFTS; MITOCHONDRIA; TUMOR necrosis factors; INFLAMMATION
- Publication
Scientific Reports, 2015, p9851
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep09851