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- Title
Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts.
- Authors
Okuno, Daisuke; Sakamoto, Noriho; Akiyama, Yoshiko; Tokito, Takatomo; Hara, Atsuko; Kido, Takashi; Ishimoto, Hiroshi; Ishimatsu, Yuji; Tagod, Mohammed S. O.; Okamura, Haruki; Tanaka, Yoshimasa; Mukae, Hiroshi
- Abstract
Idiopathic pulmonary fibrosis is a chronic intractable lung disease, leading to respiratory failure and death. Although anti-fibrotic agents delay disease progression, they are not considered curative treatments, and alternative modalities have attracted attention. We examined the effect of human γδ T cells on collagen type I in lung fibroblasts. Collagen type I was markedly reduced in a γδ T cell number-dependent manner following treatment with γδ T cells expanded with tetrakis-pivaloxymethyl 2-(thiazole-2-ylamino) ethylidene-1,1-bisphosphonate (PTA) and interleukin-2. Collagen type I levels remained unchanged on addition of γδ T cells to the culture system through a trans-well culture membrane, suggesting that cell–cell contact is essential for reducing its levels in lung fibroblasts. Re-stimulating γδ T cells with (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) reduced collagen type I levels without cell–cell contact, indicating the existence of HMBPP-induced soluble anti-fibrotic factors in γδ T cells. Adding anti-interferon-γ (IFN-γ)-neutralizing mAb restored collagen type I levels, demonstrating that human γδ T cell-derived IFN-γ reduces collagen type I levels. Conversely, interleukin-18 augmented γδ T cell-induced suppression of collagen type I. Therefore, human γδ T cells reduce collagen levels in lung fibroblasts via two distinct mechanisms; adoptive γδ T cell transfer is potentially a new therapeutic candidate.
- Subjects
FIBROBLASTS; IDIOPATHIC pulmonary fibrosis; COLLAGEN; T cells; LUNGS; INTERLEUKIN-2
- Publication
Cells (2073-4409), 2022, Vol 11, Issue 18, pN.PAG
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells11182816