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- Title
Nucleocapsid Specific Diagnostics for the Detection of Divergent SARS-CoV-2 Variants.
- Authors
Isaacs, Ariel; Amarilla, Alberto A.; Aguado, Julio; Modhiran, Naphak; Albornoz, Eduardo A.; Baradar, Alireza A.; McMillan, Christopher L. D.; Choo, Jovin J. Y.; Idris, Adi; Supramaniam, Aroon; McMillan, Nigel A. J.; Muller, David A.; Young, Paul R.; Woodruff, Trent M.; Wolvetang, Ernst J.; Chappell, Keith J.; Watterson, Daniel
- Abstract
Since the start of the COVID-19 pandemic, multiple waves of SARS-CoV-2 variants have emerged. Of particular concern is the omicron variant, which harbors 28 mutations in the spike glycoprotein receptor binding and N-terminal domains relative to the ancestral strain. The high mutability of SARS-CoV-2 therefore poses significant hurdles for development of universal assays that rely on spike-specific immune detection. To address this, more conserved viral antigens need to be targeted. In this work, we comprehensively demonstrate the use of nucleocapsid (N)-specific detection across several assays using previously described nanobodies C2 and E2. We show that these nanobodies are highly sensitive and can detect divergent SARS-CoV-2 ancestral, delta and omicron variants across several assays. By comparison, spike-specific antibodies S309 and CR3022 only disparately detect SARS-CoV-2 variant targets. As such, we conclude that N-specific detection could provide a standardized universal target for detection of current and emerging SARS-CoV-2 variants of concern.
- Subjects
SARS-CoV-2; SARS-CoV-2 Omicron variant; SARS-CoV-2 Delta variant; VIRAL antigens; IMMUNOGLOBULINS
- Publication
Frontiers in Immunology, 2022, Vol 13, p1
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2022.926262