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- Title
Simultaneous Determination of Glutamate, Glycine, and Alanine in Human Plasma Using Precolumn Derivatization with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate and High-Performance Liquid Chromatography.
- Authors
Qing Zhong Li; Qing Xian Huang; Shu Cui Li; Mei Zi Yang; Bin Rao
- Abstract
A simple, sensitive and reproducible high-performance liquid chromatography (HPLC) method has been validated for determining concentrations of glutamate, glycine, and alanine in human plasma. Proteins in plasma were precipitated with perchloric acid, followed by derivatization with 6-aminoquinolyl- N-hydroxysuccinimidyl carbamate (AQC). Simultaneous analysis of glutamate, glycine, and alanine is achieved using reversed-phase HPLC conditions and ultraviolet detection. Excellent linearity was observed for these three amino acids over their concentration ranges with correlation coefficients (r)>0.999. The intra- and inter-day precision were below 10%. This method utilizes quality control samples and demonstrates excellent plasma recovery and accuracy. The developed method has been successfully applied to measure plasma glutamate, glycine, and alanine in twenty volunteers.
- Subjects
LIQUID chromatography; AMINO acids; CARBAMATES; GLYCINE; PERCHLORIC acid; ULTRAVIOLET detectors
- Publication
Korean Journal of Physiology & Pharmacology, 2012, Vol 16, Issue 5, p355
- ISSN
1226-4512
- Publication type
Article
- DOI
10.4196/kjpp.2012.16.5.355