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- Title
A novel non-invasive optical method for quantitative visualization of pH dynamics in the rhizosphere of plants.
- Authors
BLOSSFELD, STEPHAN; GANSERT, DIRK
- Abstract
A novel optical method for non-invasive, quantitative and high-resolution imaging of spatial and temporal pH dynamics in soils mediated by plant roots is introduced. This method overcomes present limitations of measurement of pH, mainly short-term and punctiform measurements, by recording long-term dynamics of the micro-pattern of pH in the root–soil interface without disturbance of the biological and physico-chemical conditions. Juncus effusus L., rooting in a permanently flooded rhizotron, was selected as the test organism for qualifying the technique. The measurements showed pronounced diurnal variations of pH along the roots, particularly along the elongation zone. Diurnal oscillation of pH caused by the roots reached up to 0.5 units. Long-term records at 4 s intervals over more than 8 weeks revealed considerable spatial and temporal patterns of pH dynamics in the rhizosphere of about 10% of the pH scale (pH 7.0–8.5). The measured data were validated by the use of pH electrodes. Concomitantly measured oxygen concentration showed hypoxic conditions around root tips (10–70 µmol O2 L−1) and almost anoxic conditions (0.9 µmol O2 L−1) in the bulk soil. The present study qualifies this novel pH-sensing technique as a powerful analytical tool for quantitative visualization of undisturbed bioprocess dynamics.
- Subjects
HYDROGEN-ion concentration measurement; JUNCUS effusus; IMAGING systems in biology; PLANT root physiology; OPTICAL images; RHIZOSPHERE; SOIL acidity; ANALYTICAL biochemistry; SOIL biology
- Publication
Plant, Cell & Environment, 2007, Vol 30, Issue 2, p176
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1111/j.1365-3040.2006.01616.x