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- Title
Indian Trichophyton mentagrophytes squalene epoxidase erg1 double mutants show high proportion of combined fluconazole and terbinafine resistance.
- Authors
Burmester, Anke; Hipler, Uta‐Christina; Uhrlaß, Silke; Nenoff, Pietro; Singal, Archana; Verma, Shyam B.; Elsner, Peter; Wiegand, Cornelia
- Abstract
Summary: Background: The Indian ITS genotype VIII Trichophyton mentagrophytes population shows a high amount of different erg1 (ergosterol) mutants encoding for squalene epoxidase, which catalyses the first step of ergosterol biosynthesis. Objectives: Illumination of the implication of point mutations at position Ala448Thr in single and double erg1 T mentagrophytes mutants because mutants of this type were abundantly found within the Indian fungal population. Methods: Growth in fluconazole or terbinafine containing medium was analysed using a microplate‐laser‐nephelometry (MLN)‐based growth assay. Results: Ala 448 Thr erg1 single mutants were terbinafine sensitive, but about 50% of isolates showed an increased fluconazole resistance, whereas 95% of the double mutants (Phe 397 Leu, Ala 448Thr) demonstrated combined terbinafine and increased fluconazole resistance. Conclusion: The new Indian T mentagrophytes populations show several point mutations in erg1. Point mutations at position 397 were previously described and cause terbinafine resistance. A large part of the double mutants exhibit resistance to terbinafine and fluconazole, demonstrating a selective advantage of the combination of both mutations.
- Subjects
TRICHOPHYTON; FLUCONAZOLE; TERBINAFINE; ERGOSTEROL; RIBAVIRIN; BIOSYNTHESIS; THREONINE; GENOTYPES
- Publication
Mycoses, 2020, Vol 63, Issue 11, p1175
- ISSN
0933-7407
- Publication type
Article
- DOI
10.1111/myc.13150