We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cordycepin induces apoptosis of human acute monocytic leukemia cells via downregulation of the ERK/Akt signaling pathway.
- Authors
YUE WANG; HUIMIN MO; JUN GU; KAN CHEN; ZHIHUA HAN; YI LIU
- Abstract
The aim of the present study was to examine the apoptotic effect of cordycepin (COR) on human THP-1 acute monocytic leukemia cells. THP-1 cells were exposed to different concentrations of COR for 24, 48, 72 or 96 h. The cell viability and apoptotic rate were analyzed. The gene expression of Akt1, Akt2, Akt3, B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) were assessed by reverse-transcription quantitative PCR. Western blot analysis was used to detect the protein levels of phosphorylated (p)-Akt, p-extracellular signal-regulated kinase (ERK) and cleaved caspase-3. It was found that the viability of THP-1 cells was inhibited by COR in a dose- and time-dependent manner. After treatment with 200 μM COR for 24 h, the percentage of apoptotic cells was significantly increased. COR also downregulated the levels of Bcl-2, Akt1, Akt2 and Akt3, and elevated the expression of Bax. The protein levels of p-Akt and p-ERK were suppressed and cleaved caspase-3 was increased after treatment of COR. In conclusion, COR was found to induce apoptosis of THP-1 acute monocytic leukemia cells through downregulation of ERK/Akt signaling.
- Subjects
MONOCYTIC leukemia; ADENOSINE derivatives; APOPTOSIS; GENE expression; CASPASES; LEUKEMIA treatment
- Publication
Experimental & Therapeutic Medicine, 2017, Vol 14, Issue 4, p3067
- ISSN
1792-0981
- Publication type
Article
- DOI
10.3892/etm.2017.4855