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- Title
Adipose Gene Expression Profiles Related to Metabolic Syndrome Using Microarray Analyses in Two Different Models.
- Authors
Hye Jin Yoo; Hwan-Jin Hwang; Tae Woo Jung; Ja Young Ryu; Ho Cheol Hong; Hae Yoon Choi; Sei Hyun Baik; Kyung Mook Choi
- Abstract
Background: Peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist has a wide-ranging influence on multiple components of metabolic syndrome. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a useful animal model of metabolic syndrome. To determine genes related to metabolic syndrome, we examined overlapping genes that are simultaneously decreased by PPAR-γ agonists and increased in OLETF rats using microarrays in two different models. Methods: In the first microarray analysis, PPAR-γ agonist-treated db/db mice were compared to standard diet-fed db/db mice. In the second microarray analysis, OLETF rats were compared to Long-Evans Tokushima Otsuka (LETO) rats (control of OLETF rats). Results: Among the overlapping genes, in the present study, we validated that lipocalin-2 expression was significantly decreased in the visceral adipose tissue of PPAR-γ agonist-treated db/db mice compared to standard diet-fed db/db mice and increased in OLETF rats compared to LETO rats using real time reverse transcription polymerase chain reaction. Furthermore, we showed for the first time that lipocalin-2 expression was significantly increased in the visceral adipose tissues of obese humans compared with nonobese humans. In addition, the expression level of lipocalin-2 in human visceral adipose tissue had a significant positive correlation with body mass index, serum interleukin-6, adipocyte fatty acid binding protein levels, and white blood cell count. Conclusion: Lipocalin-2 was confirmed to be a significant adipokine affected by PPAR-γ agonist and obesity in the present study. Also, for the first time in human visceral adipose tissue, it was determined that the expression of lipocalin-2 from obese humans was significantly increased and correlated with circulating inflammatory markers.
- Subjects
GENE expression profiling; METABOLIC syndrome; PROTEIN microarrays; LIPOCALIN-1; ADIPOSE tissues; PEROXISOME proliferator-activated receptors; LABORATORY rats
- Publication
Diabetes & Metabolism Journal, 2014, Vol 38, Issue 5, p356
- ISSN
2233-6079
- Publication type
Article
- DOI
10.4093/dmj.2014.38.5.356