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- Title
Diversity and phylogeny of resveratrol-producing culturable endophytic fungi from Vitis species in India.
- Authors
Dwibedi, Vagish; Saxena, Sanjai
- Abstract
In the present study, resveratrol producing culturable endophytes were recovered from the Indian vineyards. Of the 145 endophytic fungi recovered, only 30.3% were positive for resveratrol production in the preliminary screening. Culture broth of only 13 isolates exhibited the actual presence of resveratrol in the range of 4.4–52.3 μg/ml by HPLC quantification. The most potent endophytic isolate producing the highest extracellular resveratrol was #19VVLPM isolated from the Pinot Noir variety. Further LC–MS analysis confirmed the similarity of fungal resveratrol with standard resveratrol. The Merlot variety exhibited the highest colonization frequency of the resveratrol-producing fungal endophytes. Using morphological methods as well as molecular phylogeny #19VVLPM was identified as Fusarium equiseti. It is a promising candidate for further development into a commercial strain.
- Subjects
INDIA; ENDOPHYTIC fungi; GRAPES; MOLECULAR phylogeny; PINOT noir; PHYLOGENY; RESVERATROL
- Publication
3 Biotech, 2019, Vol 9, Issue 5, pN.PAG
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-019-1712-x