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- Title
SERS-Based Microneedle Biosensor for In Situ and Sensitive Detection of Tyrosinase.
- Authors
Gu, Zimeng; Zhao, Di; He, Hongyan; Wang, Zhenhui
- Abstract
Tyrosinase (TYR) emerges as a key enzyme that exerts a regulatory influence on the synthesis of melanin, thereby assuming the role of a critical biomarker for the detection of melanoma. Detecting the authentic concentration of TYR in the skin remains a primary challenge. Distinguished from ex vivo detection methods, this study introduces a novel sensor platform that integrates a microneedle (MN) biosensor with surface-enhanced Raman spectroscopy (SERS) technology for the in situ detection of TYR in human skin. The platform utilized dopamine (DA)-functionalized gold nanoparticles (Au NPs) as the capturing substrate and 4-mercaptophenylboronic acid (4-MPBA)-modified silver nanoparticles (Ag NPs) acting as the SERS probe. Here, the Au NPs were functionalized with mercaptosuccinic acid (MSA) for DA capture. In the presence of TYR, DA immobilized on the MN is preferentially oxidized to dopamine quinone (DQ), a process that results in a decreased density of SERS probes on the platform. TYR concentration was detected through variations in the signal intensity emitted by the phenylboronic acid. The detection system was able to evaluate TYR concentrations within a linear range of 0.05 U/mL to 200 U/mL and showed robust anti-interference capabilities. The proposed platform, integrating MN-based in situ sensing, SERS technology, and TYR responsiveness, holds significant importance for diagnosing cutaneous melanoma.
- Subjects
PHENOL oxidase; SERS spectroscopy; BIOSENSORS; GOLD nanoparticles; MELANOGENESIS; SILVER nanoparticles
- Publication
Biosensors (2079-6374), 2024, Vol 14, Issue 4, p202
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios14040202