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- Title
Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells.
- Authors
Gonçalves, Tays Amanda Felisberto; Lima, Viviane Silva; de Almeida, Arthur José Pontes Oliveira; de Arruda, Alinne Villar; Veras, Ana Caroline Meneses Ferreira; Lima, Thaís Trajano; Soares, Evyllen Myllena Cardoso; Santos, Adhonias Correia dos; Vasconcelos, Maria Eduarda Costa de; de Almeida Feitosa, Mathania Silva; Veras, Robson Cavalcante; de Medeiros, Isac Almeida
- Abstract
Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced by endothelial progenitor cells (EPC) in hypertension. Twelve-week-old spontaneously hypertensive rats (SHR) were treated with a vehicle, carvacrol (50 or 100 mg/kg/day), or resveratrol (10 mg/kg/day) orally for four weeks. Wistar Kyoto (WKY) rats were used as the normotensive controls. Their systolic blood pressure (SBP) was measured weekly through the tail cuff. The EPCs were isolated from the bone marrow and peripherical circulation and were quantified by flow cytometry. The functionality of the EPC was evaluated after cultivation through the quantification of colony-forming units (CFU), evaluation of eNOS, intracellular detection of reactive oxygen species (ROS), and evaluation of senescence. The superior mesenteric artery was isolated to evaluate the quantification of ROS, CD34, and CD31. Treatment with carvacrol induced EPC migration, increased CFU formation and eNOS expression and activity, and reduced ROS and senescence. In addition, carvacrol reduced vascular ROS and increased CD31 and CD34 expression. This study showed that treatment with carvacrol improved the functionality of EPC, contributing to the reduction of endothelial dysfunction.
- Subjects
HYPERTENSION; ENDOTHELIAL cells; ANTIHYPERTENSIVE agents; FLOW cytometry; TERPENES; PHENOLS; CARDIOVASCULAR system physiology; ANIMAL experimentation; SYSTOLIC blood pressure; COLONY-forming units assay; MESENTERIC artery; RATS; GENE expression; STEM cells; RESEARCH funding; BONE marrow; BLOOD pressure measurement; REACTIVE oxygen species
- Publication
Nutrients, 2023, Vol 15, Issue 13, p3032
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15133032