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- Title
Aflatoxin B1 DNA-Adducts in Hepatocellular Carcinoma from a Low Exposure Area.
- Authors
Gramantieri, Laura; Gnudi, Federica; Vasuri, Francesco; Mandrioli, Daniele; Fornari, Francesca; Tovoli, Francesco; Suzzi, Fabrizia; Vornoli, Andrea; D'Errico, Antonia; Piscaglia, Fabio; Giovannini, Catia
- Abstract
Aflatoxin B1 (AFB1) is a class 1 carcinogen with an ascertained role in the development of hepatocellular carcinoma (HCC) in high exposure areas. Instead, this study aimed to assay whether chronic/intermittent, low-dose AFB1 consumption might occur in low-exposure geographical areas, ultimately accumulating in the liver and possibly contributing to liver cancer. AFB1-DNA adducts were assayed by immunostaining in liver tissues from three Italian series of twenty cirrhosis without HCC, 131 HCC, and 45 cholangiocarcinoma, and in an AFB1-induced HCC rat model. CD68, TP53 immunostaining, and TP53 RFLP analysis of R249S transversion were used to characterize cell populations displaying AFB1-DNA adducts. Twenty-five HCCs displayed AFB1-adducts both in neoplastic hepatocytes and in cells infiltrating the tumor and non-tumor tissues. Nuclear immunostaining was observed in a few cases, while most cases showed cytoplasmic immunostaining, especially in CD68-positive tumor-infiltrating cells, suggestive for phagocytosis of dead hepatocytes. Similar patterns were observed in AFB1-induced rat HCC, though with higher intensity. Cholangiocarcinoma and cirrhosis without HCC did not displayAFB1-adducts, except for one case. Despite not providing a causal relationship with HCC, these findings still suggest paying attention to detection and control measures for aflatoxins to ensure food safety in low exposure areas.
- Subjects
DNA analysis; FOOD safety; STAINS &; staining (Microscopy); PHAGOCYTOSIS; ANIMAL experimentation; CHOLANGIOCARCINOMA; RISK assessment; RATS; IMMUNOASSAY; AFLATOXINS; LIVER cells; HEPATOCELLULAR carcinoma; ENVIRONMENTAL exposure; DISEASE risk factors
- Publication
Nutrients, 2022, Vol 14, Issue 8, pN.PAG
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu14081652