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- Title
DNA Methylation Profiles of Vegans and Non-Vegetarians in the Adventist Health Study-2 Cohort.
- Authors
Miles, Fayth L.; Mashchak, Andrew; Filippov, Valery; Orlich, Michael J.; Duerksen-Hughes, Penelope; Chen, Xin; Wang, Charles; Siegmund, Kimberly; Fraser, Gary E.
- Abstract
We sought to determine if DNA methylation patterns differed between vegans and non-vegetarians in the Adventist Health Study-2 cohort. Genome-wide DNA methylation derived from buffy coat was profiled in 62 vegans and 142 non-vegetarians. Using linear regression, methylation of CpG sites and genes was categorized or summarized according to various genic/intergenic regions and CpG island-related regions, as well as the promoter. Methylation of genes was measured as the average methylation of available CpG's annotated to the nominated region of the respective gene. A permutation method defining the null distribution adapted from Storey et al. was used to adjust for false discovery. Differences in methylation of several CpG sites and genes were detected at a false discovery rate < 0.05 in region-specific and overall analyses. A vegan diet was associated predominantly with hypomethylation of genes, most notably methyltransferase-like 1 (METTL1). Although a limited number of differentially methylated features were detected in the current study, the false discovery method revealed that a much larger proportion of differentially methylated genes and sites exist, and could be detected with a larger sample size. Our findings suggest modest differences in DNA methylation in vegans and non-vegetarians, with a much greater number of detectable significant differences expected with a larger sample.
- Subjects
GENE expression; GENETIC mutation; REGRESSION analysis; VEGETARIANISM; DNA methylation; EPIGENOMICS
- Publication
Nutrients, 2020, Vol 12, Issue 12, p3697
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu12123697