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- Title
Safety assessment of Lactobacillus reuteri IDCC 3701 based on phenotypic and genomic analysis.
- Authors
Lee, Bo Som; Ban, O-Hyun; Bang, Won Yeong; Chae, Seung A; Oh, Sangki; Park, Chanmi; Lee, Minjee; Kim, Soo-Jung; Yang, Jungwoo; Jung, Young Hoon
- Abstract
Purpose: Lactobacillus reuteri is one of the most largely studied human-commensal bacteria and widely used as a form of probiotics. Safety of probiotics has become increasingly important for human consumption due to increasing health-concerns in food industry. In this study, the safety of L. reuteri IDCC 3701 isolated from human breast milk was thoroughly investigated. Methods: Whole-genome sequence analysis was performed to identify antibiotic resistance and toxigenicity of L. reuteri IDCC 3701. Phenotypic analysis such as minimal inhibitory concentration, β-hemolysis, extracellular enzyme activity, and the production of biological amines and L/D-lactate, was investigated. Finally, acute oral toxicity test was performed to access L. reuteri IDCC 3701 safety. Results: Although multiple resistances to gentamicin and kanamycin were observed in L. reuteri IDCC 3701, it was revealed that these resistances are intrinsic and not transferable through whole-genome analysis. In addition, various phenotypic analysis concerning hemolysis, enzyme activity, and D-lactate production did not show any negative results. Although L. reuteri IDCC 3701 harbors a histidine decarboxylase gene, no biogenic amines were detected. Finally, L. reuteri IDCC 3701 exhibited no evidence of acute toxicity according to an in vivo study. Conclusion: Our findings demonstrate that L. reuteri IDCC 3701 is considered to be safe for human consumption as probiotics based on the in silico, in vitro and in vivo studies.
- Publication
Annals of Microbiology, 2021, Vol 71, Issue 1, p1
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1186/s13213-021-01622-y