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- Title
Respuesta de células T, citocinas y anticuerpos frente al péptido (365-377) de la proteína de adhesión celular de Streptococcus mutans.
- Authors
Roa Molina, Nelly Stella; Gómez Ramírez, Soledad Isabel; Rodríguez Ciódaro, Adriana
- Abstract
Background: PAc(365-377) peptide derived of Streptococcus mutans PAc protein has been used in the search for a vaccine against dental caries in humans. This peptide has T and B epitopes in animal model (mice) but this is unknown in naturally sensitized humans. Purpose: To analyze the humoral and cellular immune response to the PAc(365-377) peptide regarding dental caries status. Methods: 30 subjects were classified into three groups: active cavities, history of cavities, and caries free subjects. Blood and saliva samples were obtained from each subject. Measures included total amount of salivary and serum IgA and IgG against the PAc(365-377) peptide through ELISA and detection of functional populations of memory T cells that reacted to the PAc(365-377) peptide through frequency of IFN-g and IL-2 (TH1 profile), IL-4, IL-5, IL-10 and IL-13 (TH2 profile) with antigen-specific immune function assays through flow cytometry. Results: A memory cellular and humoral immune response against the PAc(365-377) peptide in naturally sensitized humans was observed. TH0, TH1, and TH2 profiles were detected with TH0 profile prevalence for LTCD4+ and TH1 profile prevalence for LTCD8+ in the caries free group. 54% of subjects did not react to LTCD4+ analyzed cytokines and 70% for LTCD8+. There were no statistically significant differences between the groups studied. Conclusions: Cellular and humoral immune response to the PAc(365-377) synthetic peptide from S. mutans did not show association with health or disease in naturally sensitized humans.
- Publication
Universitas Odontológica, 2014, Vol 33, Issue 71, p41
- ISSN
0120-4319
- Publication type
Article
- DOI
10.11144/Javeriana.uo33-71.rcca