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- Title
SURVEY THE EFFECT OF LICORICE EXTRACT ON AFLR GENE EXPRESSION AND AFLATOXIN PRODUCTION IN ASPERGILLUS PARASITICUS VIA REAL TIME PCR.
- Authors
Mohseni, Rashin; Noorbakhsh, Fatemeh; Omran, Ayatolah Nasrollahi; Moazeni, Maryam; Rezaie, Sasan
- Abstract
Introduction and Objectives: Aflatoxin is a fungal metabolite from Aspergillus species that is toxigenic, mutagenic, carcinogenic, immunosuppressive and even teratogenic in human and various species of animals. So the aflatoxin is important in food industries, animal husbandry and medical area and there are enormous economical detriments due to this toxin. These factors resulted in many studies on the extracts and plant compounds to reduce the growth of aflatoxin producing organisms, inhibit the toxin production and also suppress the major toxin encoded genes like aflR in these organisms. Licorice is an important medicinal plant in our traditional medicine and it posses many antimicrobial activities. There is no report around the licorice effects or its mechanism on the aflatoxin producing Aspergillus species. The present study focuses on the licorice extract inhibitory effects on the aflR gene expression and the growth and survival of Aspergillus parasiticus. Materials and Methods: After the culture of A. parasiticus in the toxin inducer medium, the minimal inhibitory concentration and minimal fungicidal concentrations for licorice extract was measured. The aflatoxin concentration in the control and treated media was determined using HPLC method. Also after harvesting the fungi from toxin inducing medium its mRNA was extracted and the cDNA was synthesized by universal primers. The quantitative change in the aflR expression was analyzed via real time PCR. The statistical analysis was performed by SPSS program. Results: The production of fungal mycelium was decreased by increasing the concentration of licorice extract. The minimum fungicide concentration was 500mg/ml of the extract. The HPLC analysis revealed that the 10mg/ml of licorice extract could inhibit the toxin production by 99. 9%. Conclusion: In this concentration aflR gene expression was suppressed up to 40% that was revealed by quantitative RT-PCR analysis.
- Subjects
LICORICE (Plant); PLANT extracts; GENE expression; AFLATOXINS; ASPERGILLUS parasiticus; IMMUNOSUPPRESSIVE agents; POLYMERASE chain reaction
- Publication
Jundishapur Journal of Microbiology, 2013, p11
- ISSN
2008-3645
- Publication type
Article