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- Title
Etomidate alleviates ovarian ischemia-reperfusion injury in rats.
- Authors
Kölükçü, Vildan; Balta, Mehtap Gürler; Tapar, Hakan; Karaman, Tuğba; Karaman, Serkan; Unsal, Velid; Gevrek, Fikret; Katar, Muzaffer
- Abstract
BACKGROUND: This study investigates the protective effects of etomidate against oxidative damage in an experimental model of ovarian ischemia-reperfusion injury. METHODS: A total of 24 female rats were randomized into three groups. Group 1 served as the control. Group 2 underwent an ovarian torsion/detorsion procedure. Group 3 underwent similar procedures as Group 2; additionally, 4 mg/kg of etomidate was administered intraperitoneally 30 minutes before ovarian detorsion. Blood samples were analyzed for lipid peroxidation, pro-inflammatory cytokine levels, and antioxidant enzyme activity.Furthermore, histopathological scoring was performed to evaluate tissue damage in the ovaries. RESULTS: Biochemical analysis of blood samples revealed reductions in pro-inflammatory cytokines, including interleukin-1 Beta (IL1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), in Group 3 compared to Group 2 (p=0.005, p=0.016, and p<0.001, respectively). Additionally, a decrease in malondialdehyde (MDA) levels was observed in Group 3 compared to Group 2 (p<0.001). In contrast, activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were significantly increased in Group 3 compared to Group 2 (p=0.031 and p=0.001, respectively). Furthermore, Group 3 demonstrated notable reductions in histopathological scores for follicular degeneration, vascular occlusion, bleeding, and inflammation compared to Group 2 (p<0.001, p<0.001, p<0.001, and p=0.001, respectively). CONCLUSION: Etomidate alleviates ischemia-reperfusion injury in a rat ovarian torsion-detorsion model by improving both histopathological and biochemical outcomes.
- Subjects
ANTIOXIDANT analysis; REPERFUSION injury; TREATMENT effectiveness; DESCRIPTIVE statistics; OXIDATIVE stress; ETOMIDATE; RATS; LIPID peroxidation (Biology); ANIMAL experimentation; CYTOKINES; GLUTATHIONE peroxidase; OVARIES; INTERLEUKINS; TUMOR necrosis factors; MALONDIALDEHYDE
- Publication
Turkish Journal of Trauma & Emergency Surgery / Ulusal Travma ve Acil Cerrahi Dergisi, 2024, Vol 30, Issue 6, p375
- ISSN
1306-696X
- Publication type
Article
- DOI
10.14744/tjtes.2024.27388