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- Title
Electron transport chain dysfunction by HO is linked to increased reactive oxygen species production and iron mobilization by lipoperoxidation: studies using Saccharomyces cerevisiae mitochondria.
- Authors
Cortés-Rojo, Christian; Estrada-Villagómez, Mirella; Calderón-Cortés, Elizabeth; Clemente-Guerrero, Mónica; Mejía-Zepeda, Ricardo; Boldogh, Istvan; Saavedra-Molina, Alfredo
- Abstract
The mitochondrial electron transport chain (ETC) contains thiol groups (−SH) which are reversibly oxidized to modulate ETC function during HO overproduction. Since deleterious effects of HO are not limited to -SH oxidation, due to the formation of other HO-derived species, some processes like lipoperoxidation could enhance the effects of HO over ETC enzymes, disrupt their modulation by -SH oxidation and increase superoxide production. To verify this hypothesis, we tested the effects of HO on ETC activities, superoxide production and iron mobilization in mitochondria from lipoperoxidation-resistant native yeast and lipoperoxidation-sensitized yeast. Only complex III activity from lipoperoxidation-sensitive mitochondria exhibited a higher susceptibility to HO and increased superoxide production. The recovery of ETC activity by the thiol reductanct β-mercaptoethanol (BME) was also altered at complex III, and a role was attributed to lipoperoxidation, the latter being also responsible for iron release. A hypothetical model linking lipoperoxidation, increased complex III damage, superoxide production and iron release is given.
- Subjects
REACTIVE oxygen species; ELECTRON transport; SACCHAROMYCES cerevisiae; IRON; MITOCHONDRIA
- Publication
Journal of Bioenergetics & Biomembranes, 2011, Vol 43, Issue 2, p135
- ISSN
0145-479X
- Publication type
Article
- DOI
10.1007/s10863-011-9339-6