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- Title
Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library.
- Authors
Mena, Marco A.; Treynor, Thomas P.; Mayo, Stephen L.; Daugherty, Patrick S.
- Abstract
The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.
- Subjects
GREEN fluorescent protein; IMAGING compatibility of biomedical materials; IMAGE quality in imaging systems; COMPUTER algorithms; FLUORIMETRY; BIOTECHNOLOGY research
- Publication
Nature Biotechnology, 2006, Vol 24, Issue 12, p1569
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt1264