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- Title
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
- Authors
Schmitt-Ulms, Gerold; Hansen, Kirk; Jialing Liu; Cowdrey, Cynthia; Jian Yang; DeArmond, Stephen J; Cohen, Fred E; Prusiner, Stanley B; Baldwin, Michael A
- Abstract
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the ?-secretase complex in high salt concentrations and detergents and was compatible with mass spectrometric identification of cross-linked aph-1, presenilin-1 and nicastrin. We then applied tcTPC to identify more than 20 proteins residing in the vicinity of the cellular prion protein (PrPC), suggesting that PrP is embedded in specialized membrane regions with a subset of molecules that, like PrP, use a glycosylphosphatidylinositol anchor for membrane attachment. Many of these proteins have been implicated in cell adhesion/neuritic outgrowth, and harbor immunoglobulin C2 and fibronectin type III-like motifs.
- Subjects
IMMUNOGLOBULINS; PROTEINS; CLEANING compounds; ADHESION; TISSUES; PLASMA cells; PRESENILINS
- Publication
Nature Biotechnology, 2004, Vol 22, Issue 6, p724
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt969