We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Telomerized human microvasculature is functional in vivo.
- Authors
Yang, Jiwei; Nagavarapu, Usha; Relloma, Kenneth; Sjaastad, Michael D.; Moss, William C.; Passaniti, Antonino; Herron, G. Scott
- Abstract
Previously we showed the superior in vitro survival of human telomerase reverse transcriptase (hTERT)-transduced human endothelial cells (EC). Here we show that retroviral-mediated transduction of hTERT in human dermal microvascular EC (HDMEC) results in cell lines that form microvascular structures when subcutaneously implanted in severe combined immunodeficiency (SCID) mice. Anti-human type IV collagen basement membrane immunoreactivity and visualization of enhanced green fluorescent protein (eGFP)-labeled microvessels confirmed the human origin of these capillaries. No human vasculature was observed after implantation of HT1080 fibrosarcoma cells, 293 human embryonic kidney cells, or human skin fibroblasts. Intravascular red fluorescent microspheres injected into host circulation were found within green "telomerized" microvessels, indicating functional murine-human vessel anastamoses. Whereas primary HDMEC-derived vessel density decreased with time, telomerized HDMEC maintained durable vessels six weeks after xenografting. Modulation of implant vessel density by exposure to different angiogenic and angio-static factors demonstrated the utility of this system for the study of human microvascular remodelingin vivo.
- Subjects
TELOMERASE; GENETIC transduction
- Publication
Nature Biotechnology, 2001, Vol 19, Issue 3, p219
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/85655