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- Title
Downregulation of stress-associated protein 1 (<italic>PagSAP1</italic>) increases salt stress tolerance in poplar (<italic>Populus alba</italic> × <italic>P. glandulosa</italic>).
- Authors
Yoon, Seo-Kyung; Bae, Eun-Kyung; Lee, Hyoshin; Choi, Young-Im; Han, Muho; Choi, Hyunmo; Kang, Kyu-Suk; Park, Eung-Jun
- Abstract
Key message: <bold>Downregulating</bold><bold><italic>PagSAP1</italic></bold><bold>expression increases salt tolerance in poplar and leads to the alterations in the regulation of genes involved in maintaining cellular ionic homeostasis.</bold>Abstract: Plants have evolved defense mechanisms to cope with unfavorable climate conditions such as salinity, drought, and extreme temperatures. Such adaptive strategies include fine-tuning of stress-associated proteins (SAPs). Here, we investigated the molecular and physiological characteristics of the novel gene <italic>PagSAP1</italic> from hybrid poplar (<italic>Populus alba</italic> × <italic>P. glandulosa</italic>) in response to salt stress. <italic>PagSAP1</italic> encodes a putative protein containing an A20 and AN1 zinc-finger domain at its N- and C-terminus, respectively. Salt stress significantly downregulated the expression of <italic>PagSAP1</italic> in roots, but not leaves, of poplar at the seven-leaf stage. Compared to control plants, knockdown (RNAi) transgenic poplar plants showed strong tolerance to salt stress, while overexpression (OX) of <italic>PagSAP1</italic> increased salt sensitivity. Analysis of Ca2+, Na+, and K+ contents in roots and leaves following salt treatment (150 mM NaCl) revealed that RNAi plants accumulated more Ca2+ and K+, and less Na+ than <italic>PagSAP1</italic> OX plants. The RNAi lines exhibited increased expression of genes involved in maintaining cellular ionic homeostasis across the plasma membrane, such as salt overly sensitive 3 (<italic>SOS3</italic>), <italic>SOS1</italic>, high-affinity K+ transporter 1 (<italic>HKT1</italic>), <italic>H</italic>+-<italic>ATPase</italic>, <italic>AAA</italic>-<italic>type ATPase</italic>, and Arabidopsis K+ channel 2 (<italic>AKT2</italic>). Taken together, these results indicate that <italic>PagSAP1</italic> represents a promising candidate gene for engineering trees with improved salt tolerance, which would increase their suitability for planting in marginal lands, such as reclaimed areas.
- Subjects
PLANT physiology; SOIL salinity; GENE expression; CELL membranes; ARABIDOPSIS proteins
- Publication
Trees: Structure & Function, 2018, Vol 32, Issue 3, p823
- ISSN
0931-1890
- Publication type
Article
- DOI
10.1007/s00468-018-1675-2