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- Title
Excitotoxic Death of Retinal Neurons In Vivo Occurs via a Non-Cell-Autonomous Mechanism.
- Authors
Lebrun-Julien, Frédéric; Duplan, Laure; Pernet, Vincent; Osswald, Ingrid; Sapieha, Przemyslaw; Bourgeois, Philippe; Dickson, Kathleen; Bowie, Derek; Barker, Philip A.; di Polo, Adriana
- Abstract
The central hypothesis of excitotoxicity is that excessive stimulation of neuronal NMDA-sensitive glutamate receptors is harmful to neurons and contributes to a variety of neurological disorders. Glial cells have been proposed to participate in excitotoxic neuronal loss, but their precise role is defined poorly. In this in vivo study, we show that NMDA induces profound nuclear factor κB (NF-κB) activation in Müller glia but not in retinal neurons. Intriguingly, NMDA-induced death of retinal neurons is effectively blocked by inhibitors of NF-κB activity. We demonstrate that tumor necrosis factor α (TNFα) protein produced in Müller glial cells via an NMDA-induced NF-κB-dependent pathway plays a crucial role in excitotoxic loss of retinal neurons. This cell loss occurs mainly through a TNFκ-dependent increase in Ca2+-permeable AMPA receptors on susceptible neurons. Thus, our data reveal a novel non-cell-autonomous mechanism by which glial cells can profoundly exacerbate neuronal death following excitotoxic injury.
- Subjects
RETINOBLASTOMA; TUMOR necrosis factors; CYTOKINES; GROWTH factors; NEUROGLIA; NERVE tissue; NERVOUS system
- Publication
Journal of Neuroscience, 2009, Vol 29, Issue 17, p5536
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.0831-09.2009