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- Title
Quantitative evaluation of bacteria adherent to polyelectrolyte HEMA-based hydrogels.
- Authors
Francesca Berlutti; Franco Rosso; Pietro Bosso; Francesco Giansanti; Maria Ajello; Alfredo De Rosa; Ernesto Farina; Giovanni Antonini; Piera Valenti
- Abstract
The use of adhesive poly(HEMA)-based hydrogels is standard practice in dental restorative procedures. Microorganisms, which potentially can cause oral pathologies, may colonize these polymers. In the present work, bacterial adhesion to polymers prepared with 2-hydroxyethyl methacrylate (HEMA) and to different molar ratios of 2-acrylamido-2-methylpropane-sulfonic acid (AMPS) and/or to 2-methacryloyloxyethyl-tri-methyl-ammonium chloride (METAC) co-monomers were tested. A colorimetric assay system that utilizes the Microbo revelation medium (Microbo srl, Rome, Italy) for microbial counts is shown to be capable of counting the number of adherent bacterial cells without removing them from polymer surfaces. In conditions that mimic those present in the oral cavity, similar bacterial adhesion percentages on the same polymer were observed with the different bacteria belonging to both gram-positive and gram-negative genera, such as Streptococcus sobrinus and Streptococcus oralis (resident microorganisms in the oral cavity) and Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa (transient microorganisms in the oral cavity). It is determined that the physico-chemical characteristics of poly(HEMA)-based hydrogels are the major factors promoting bacterial adhesion, which increased with increasing water content in the swollen polymers, reaching maximal values on the cationic polymers. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1825, 2003
- Subjects
HYDROGELS; DENTAL fillings; PATHOLOGY; BACTERIAL adhesion
- Publication
Journal of Biomedical Materials Research, Part A, 2003, Vol 67, Issue 1, p18
- ISSN
1549-3296
- Publication type
Article
- DOI
10.1002/jbm.a.10026