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- Title
Hyperinsulinemia decreases second-phase but not first-phase arginine-induced insulin release in humans.
- Authors
Piatti, Piero M.; Pontiroli, Antonio E.; Caumo, Andrea; Santambrogio, Giovanni; Monti, Lucilla D.; Costa, Sabrina; Garbetta, Francesca; Baruffaldi, Laura; Cobelli, Claudio; Pozza, Guido; Piatti, P M; Pontiroli, A E; Caumo, A; Santambrogio, G; Monti, L D; Costa, S; Garbetta, F; Baruffaldi, L; Cobelli, C; Pozza, G
- Abstract
The aim of this study was to investigate the effect of hyperinsulinemia on the first and second phase of arginine-induced insulin release in humans. Seven healthy subjects underwent three studies (lasting 360 min): a control study using saline infusion and two euglycemic clamps using a low-dose (0.33 mU.kg-1.min-1) and a high-dose (1.20 mU.kg-1.min-1) insulin infusion. After a 3-h equilibration period, arginine (25 g) was infused for 30 min, and insulin and C-peptide responses to arginine were followed for 180 min. At the end of the equilibration period, before arginine administration, steady-state insulin levels were (means +/- SE) 60.0 +/- 2.4, 165.6 +/- 1.8, and 455.4 +/- 7.8 pmol/l during saline, low-dose, and high-dose insulin infusions, respectively. The time course of insulin release during the arginine test was calculated from C-peptide concentrations by using C-peptide kinetic modeling and deconvolution. In particular, first-phase and second-phase insulin response was obtained by integrating the time course of the insulin release during either the first 5 min or the following 40 min of the arginine test, respectively. Whereas first-phase insulin release was independent of any effect induced by either insulin infusion, second-phase insulin release was reduced in a similar degree by both insulin infusion doses. First phase was 75.5 +/- 10.1, 73.7 +/- 12.8, and 73.4 +/- 10.3 pmol/kg, whereas second phase was 266.1 +/- 46.0, 143.1 +/- 33.5, and 133.0 +/- 30.2 pmol/kg for saline, low-dose, and high-dose insulin infusions, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
- Publication
Diabetes, 1994, Vol 43, Issue 9, p1157
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.43.9.1157