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- Title
Automated method for isolation of human pancreatic islets.
- Authors
Ricordi, Camillo; Lacy, Paul E.; Finke, Edward H.; Olack, Barbara J.; Scharp, David W.; Ricordi, C; Lacy, P E; Finke, E H; Olack, B J; Scharp, D W
- Abstract
We describe an automated method for the isolation of human pancreatic islets. The procedure meets the following requirements: 1) minimal traumatic action on the islets, 2) continuous digestion in which the islets that are progressively liberated can be saved from further enzymatic action, 3) minimal human intervention in the digestion process, and 4) high yield and purity of the isolated islets. After purification on Ficoll gradients, an average of 164,600 islets/pancreas was obtained (2279 islets/g), with an average purity of 78.5% islets. The average volume and average insulin content of the final islet preparation were 348 mm3 and 93.4 U, respectively. The islets were morphologically intact with a normal degree of beta-granulation and responded to glucose stimulation with a fivefold increase of insulin secretion over basal levels. The procedure is now being used for the initiation of the second phase of clinical trials on human islet transplants.
- Subjects
CELL separation; COMPARATIVE studies; INSULIN; ISLANDS of Langerhans; RESEARCH methodology; MEDICAL cooperation; PERFUSION; RESEARCH; RESEARCH funding; EVALUATION research
- Publication
Diabetes, 1988, Vol 37, Issue 4, p413
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.37.4.413