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- Title
Phenylephrine enhances glutamate release in the medial prefrontal cortex through interaction with N-type Ca<sup>2+</sup> channels and release machinery.
- Authors
Luo, Fei; Li, Si‐hai; Tang, Hua; Deng, Wei‐ke; Zhang, Yu; Liu, Ying
- Abstract
α1-adrenoceptors (α1-ARs) stimulation has been found to enhance excitatory processes in many brain regions. A recent study in our laboratory showed that α1-ARs stimulation enhances glutamatergic transmission via both pre- and post-synaptic mechanisms in layer V/VI pyramidal cells of the rat medial prefrontal cortex ( mPFC). However, a number of pre-synaptic mechanisms may contribute to α1-ARs-induced enhancement of glutamate release. In this study, we blocked the possible post-synaptic action mediated by α1-ARs to investigate how α1-ARs activation regulates pre-synaptic glutamate release in layer V/VI pyramidal neurons of mPFC. We found that the α1-ARs agonist phenylephrine (Phe) induced a significant enhancement of glutamatergic transmission. The Phe-induced potentiation was mediated by enhancing pre-synaptic glutamate release probability and increasing the number of release vesicles via a protein kinase C-dependent pathway. The mechanisms of Phe-induced potentiation included interaction with both glutamate release machinery and N-type Ca2+ channels, probably via a pre-synaptic Gq/phospholipase C/protein kinase C pathway. Our results may provide a cellular and molecular mechanism that helps explain α1-ARs-mediated influence on PFC cognitive functions.
- Subjects
PHENYLEPHRINE; PREFRONTAL cortex; GLUTAMIC acid; EXCITATORY amino acid agents; NEURAL transmission; LABORATORY rats; CALCIUM channels
- Publication
Journal of Neurochemistry, 2015, Vol 132, Issue 1, p38
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/jnc.12941