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- Title
Retrovirally transduced NCAM140 facilitates neuronal fate choice of hippocampal progenitor cells.
- Authors
Ju Hee Kim; Jung-Ha Lee; Jin-Yong Park; Chang-Hwan Park; Chae-Ok Yun; Sang-Hun Lee; Yong-Sung Lee; Hyeon Son
- Abstract
Neural cell adhesion molecule (NCAM) influences proliferation and differentiation of neuronal cells. However, only a little is known about the downstream effects of NCAM signalling, such as alterations in gene transcription, which are associated with cell fate choice. To examine whether NCAM plays a role in cell fate choice during hippocampal neurogenesis, we performed a gain-of-function study, using a retroviral vector which contained full-length NCAM140 cDNA and the marker gene EGFP , and found that NCAM140 promoted neurogenesis by activating proneural transcription activators with concurrent inhibition of gliogenesis. The enhanced transcript levels of proneural transcription factors in NCAM140-transduced cells were down-regulated by treatment of the cells with mitogen-activated protein kinase kinase (MEK) inhibitor PD098059. Overall, these findings suggest that NCAM140 may facilitate hippocampal neurogenesis via regulation of proneurogenic transcription factors in an extracellular signal-regulated kinase (ERK)-dependent manner.
- Subjects
HIPPOCAMPUS (Brain); CEREBRAL cortex; LIMBIC system; GENETIC transcription; PROTEIN kinases; DEVELOPMENTAL neurobiology
- Publication
Journal of Neurochemistry, 2005, Vol 94, Issue 2, p417
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2005.03208.x