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- Title
Effect of hydrogen sulfide on PC12 cell injury induced by high ATP concentration.
- Authors
Kunli Yang; Hu Luo; Yajie Liu; Yankai Ren; Meixia Guo; Bin Wang; Chih-huang Yang; Dongliang Li
- Abstract
Purpose: To investigate the potential protective effect of hydrogen sulfide against neural cell damage induced by a high-concentration of adenosine triphosphate (ATP). Methods: PC12 cells were incubated with ATP in order to induce cell damage. The extracellular level of H2S and protein expression of cystathionine-ß-synthase (CBS) were determined. The PC12 cells pretreated with NaHS, aminooxyacetic acid (AOAA) and KN-62, prior to further incubation with ATP, and the effect of the treatments on cell viability was investigated. Results: High-concentration ATP induced cell death in PC12 cells, and this was accompanied by markedly increased contents of extracellular H2S and CBS expression (p < 0.05). The ATP-induced cytotoxicity was significantly compromised after pretreatment with H2S. (p < 0.05). The viability of PC12 cells pretreated with NaHS and AOAA was significantly higher than that of PC12 cells treated with ATP alone. In addition, the viability of ATP-treated PC12 cells was further markedly increased after pretreatment with NaHS and KN-62 (p < 0.05). Conclusion: ATP induced a concentration- and time-dependent cytotoxicity in PC12 cells via the endogenous H2S/CBS system. Supplementation with exogenous H2S mitigated the cell damage induced by high concentration of ATP via a specific mechanism which may be specifically related to P2X7R.
- Subjects
HYDROGEN sulfide; ADENOSINE triphosphate; PROTEIN expression; CELL death; CELL survival; PURINERGIC receptors; WOUNDS &; injuries
- Publication
Tropical Journal of Pharmaceutical Research, 2022, Vol 21, Issue 2, p281
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v21i2.10