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- Title
Shenxiong Drop Pill exerts neuroprotective effect against focal cerebral ischemia partly via regulation of the expressions of ICAM-1 and caspase-3.
- Authors
Shuo-guo Jin; Ze-ran Chen; Yang Zhang; Meng-yuan Huang; Meng Hou; Ning-jing Ran; Wei-yin Chen; Fang Yang; Xin-xia Zhang
- Abstract
Purpose: To investigate the effect of Shenxiong Drop Pill (SXDP) on cerebral infarction (CI) in rats, and the involvement of anti-inflammatory response in the process. Methods: Rats were sacrificed at three different time points, viz, 24, 48 and 72 h after establishment of CI model. Neurological deficit score (NDS) was determined using Bederson's neurological behavioral scoring method, whereas triphenyltetrazolium chloride (TTC) staining was used to show brain injury. The integrated optical density (IOD) of Nissl bodies and caspase-3-positive nerve cells were measured with Nissl staining and SP kit, respectively. The mRNA expression of intercellular adhesion molecule 1 (ICAM-1) was determined using reverse transcription-polymerase chain reaction (RT-PCR). Results: SXDP produced neuroprotective effect at high, medium, and low doses. The infarct volumes in the high-, medium- and low-dose SXDP, and cyclophosphamide groups were significantly reduced at each time point. Different doses of SXDP significantly reduced the mRNA expression of ICAM-1 and the IOD of caspase-3. Conclusion: These results indicate that SXDP exerts neuroprotective effects against ischemic injury by negatively regulating ICAM-1/caspase-3 downstream of inflammatory and apoptosis pathways.
- Subjects
CASPASES; CD54 antigen; CEREBRAL ischemia; GENE expression; NEUROPROTECTIVE agents; APOPTOSIS
- Publication
Tropical Journal of Pharmaceutical Research, 2021, Vol 20, Issue 10, p2015
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v20i10.1