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- Title
Genetic evidence suggests that GIS functions downstream of TCL1 to regulate trichome formation in <italic>Arabidopsis</italic>.
- Authors
Zhang, Na; Yang, Li; Luo, Sha; Wang, Xutong; Wang, Wei; Cheng, Yuxin; Tian, Hainan; Zheng, Kaijie; Cai, Ling; Wang, Shucai
- Abstract
Background: Trichome formation in Arabidopsis is regulated by a MBW complex formed by MYB, bHLH and WD40 transcriptional factors, which can activate <italic>GLABRA2</italic> (<italic>GL2</italic>) and the R3 MYB transcription factor genes. GL2 promotes trichome formation, whereas R3 MYBs are able to block the formation of the MBW complex. It has been reported that the C2H2 transcription factor GIS (GLABROUS INFLORESCENCE STEMS) functions upstream of the MBW activator complex to regulate trichome formation, and that the expression of TCL1 is not regulated by the MBW complex. However, <italic>gis</italic> and the R3 MYB gene mutant <italic>tcl1</italic> (<italic>trichomeless 1</italic>) have opposite inflorescence trichome phenotypes, but their relationship in regulating trichome formation remained unknown. Results: By generating and characterization of the <italic>gis tcl1</italic> double mutant, we found that trichome formation in the <italic>gis tcl1</italic>double and the <italic>tcl1</italic> single mutants were largely indistinguishable, but the trichome formation in the <italic>35S:TCL1</italic>/<italic>gis</italic> transgenic plant was similar to that in the <italic>gis</italic> mutant. By using quantitative RT-PCR analysis, we showed that expression level of <italic>GIS</italic> was increased in the triple mutant <italic>tcl1 try cpc</italic>, but the expression level of <italic>TCL1</italic> was not affected in the <italic>gis</italic> mutant. On the other hand, trichome morphology in both <italic>gis tcl1</italic> and <italic>35S:TCL1</italic>/<italic>gis</italic> plants was similar to that in the <italic>gis</italic> mutant. Conclusions: In summary, our results indicate that GIS may work downstream of TCL1 to regulate trichome formation, and GIS has a dominant role in controlling trichome morphology.
- Subjects
ARABIDOPSIS; ARABIDOPSIS thaliana; TRICHOMES; MORPHOGENESIS; PLANT hormones
- Publication
BMC Plant Biology, 2018, Vol 18, Issue 1, p1
- ISSN
1471-2229
- Publication type
Article
- DOI
10.1186/s12870-018-1271-z