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- Title
Cell cycle analysis of the CD133(+) and CD133(-) cells isolated from human colorectal cancer.
- Authors
Gharagozloo M; Mirzaei HR; Bagherpour B; Rezaei A; Kalantari H; Sanei MH; Hosseini M; Mohajeri G; Tabatabai A; Hashemi M; Gharagozloo, Marjan; Mirzaei, Hamid R; Bagherpour, Bahram; Rezaei, Abbas; Kalantari, Hamid; Sanei, Mohammad H; Hosseini, Mohsen; Mohajeri, Gholamreza; Tabatabai, Abbas; Hashemi, Mozaffar
- Abstract
<bold>Aim: </bold>The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary human colorectal tumors. <bold>Materials and Methods: </bold>After mechanical and enzymatic dissociation of the tumor samples, CD133(+) and CD133(-) subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133(+) and CD133(-) cells isolated from primary and liver metastatic cancer cells. <bold>Results: </bold>The results indicated that CD133(+) cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133(-) cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133(-) cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase. <bold>Conclusion: </bold>The present study provides evidence that CD133(+) cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-therapy patients.
- Publication
Journal of Cancer Research & Therapeutics, 2012, Vol 8, Issue 3, p399
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.103520