We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic profiling of aortic allografts: prothymosin alpha as potential target?
- Authors
Joosten, Simone A.; Smit van Dixhoorn, Mieneke G. A.; Borrias, Maria C.; Ham, Vanessa; Groot Koerkamp, Marian J. A.; Savolainen-Peltonen, Hanna M.; Häyry, Pekka; Daha, Mohamed R.; Kooten, Cees; Paul, Leendert C.
- Abstract
Transplant arteriosclerosis is the result of intima proliferation in large vessels upon organ transplantation. Obliteration of the vascular lumen will ultimately lead to ischemia and late graft failure. Gene array analysis was performed to identify factors involved in the pathogenesis of transplant arteriosclerosis. Aortic transplants from Dark Agouti to Wistar Furth rats were performed to identify potential target genes. Hierarchical clustering of genes specifically upregulated in allogeneic but not in syngeneic aortas revealed 19 genes. A gene that fulfilled these criteria is prothymosin alpha (PTMA), a regulator of estrogen receptor transcriptional activity. PTMA gene and protein expression levels were confirmed by PCR and immunohistochemistry. Estrogen receptor staining was increased in allogeneic aortas. Furthermore, cyclin D1 a downstream target of PTMA, was also up regulated in allogeneic aortas. In conclusion, PTMA was identified as potential candidate gene involved in transplant arteriosclerosis.
- Subjects
HOMOGRAFTS; ARTERIAL occlusions; TRANSPLANTATION of organs, tissues, etc.; BLOOD circulation; ORGAN donation; SEX hormones
- Publication
Transplant International, 2005, Vol 18, Issue 8, p1010
- ISSN
0934-0874
- Publication type
Article
- DOI
10.1111/j.1432-2277.2005.00157.x