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- Title
Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical.
- Authors
Fernández ‐ de ‐ Alba, Carlos; Takahashi, Hiroki; Richard, Alexandre; Chenavier, Yves; Dubois, Lionel; Maurel, Vincent; Lee, Daniel; Hediger, Sabine; De Paëpe, Gaël
- Abstract
Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.
- Subjects
POLARIZATION (Nuclear physics); NUCLEAR magnetic resonance spectroscopy; LIPOSOMES; LIPIDS; BIRADICALS
- Publication
Chemistry - A European Journal, 2015, Vol 21, Issue 12, p4512
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201404588