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- Title
Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption.
- Authors
Hyang Yeon Kim; Mee Youn Lee; Hye Min Park; Yoo Kyoung Park; Jong Cheol Shon; Kwang-Hyeon Liu; Choong Hwan Lee
- Abstract
In this study, we investigated the clinical changes induced by a high fat diet (HFD) and caffeine consumption in a rat model. The mean body weight of the HFD with caffeine (HFDC)-fed rat was decreased compared to that of the HFD-fed rat without caffeine. The levels of cholesterol, triglycerides (TGs), and free fatty acid, as well as the size of adipose tissue altered by HFD, were improved by caffeine consumption. To investigate the metabolites that affected the change of the clinical factors, the urine and serum of rats fed a normal diet (ND), HFD, and HFDC were analyzed using ultra performance liquid chromatography quadruple time-of-flight mass spectrometry (UPLC-Q-TOF-MS), gas chromatography (GC-TOF-MS), and linear trap quadruple mass spectrometry (LTQ-XL-MS) combined with multivariate analysis. A total of 68 and 52 metabolites were found to be different in urine and serum, respectively. After being fed caffeine, some glucuronide-conjugated compounds, lysoPCs, CEs, DGs, TGs, taurine, and hippuric acid were altered compared to the HFD group. In this study, caffeine might potentially inhibit HFD-induced obesity and we suggest possible biomarker candidates using MS-based metabolite profiling.
- Subjects
METABOLITE analysis; URINALYSIS; BLOOD serum analysis; HIGH-fat diet; CAFFEINE; LABORATORY rats
- Publication
Molecules, 2015, Vol 20, Issue 2, p3107
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules20023107