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- Title
Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71.
- Authors
Yin, Dan; Zhu, Yanan; Wang, Kaifeng; Wang, Jing; Zhang, Xiru; Han, Min; He, Yaqing; Chen, Qing; Hu, Guifang
- Abstract
Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3.767 log10 genomic copies (LGC)/reaction. The specificity was 100%. In a clinical sample evaluation, this approach demonstrated sufficient clinical performance when compared with a commercial RT-qPCR diagnostic kit. Thus, the RT-RPA assay may be a promising alternative for the detection of EV71-C4.
- Subjects
HAND, foot &; mouth disease; ENTEROVIRUSES; REVERSE transcriptase polymerase chain reaction; PERFORMANCE evaluation; PHYLOGENY
- Publication
Archives of Virology, 2018, Vol 163, Issue 9, p2459
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-018-3859-x